Fluorescence Assays in Drug Discovery
All day 8 September and morning of
9 September 2008
What will I gain by attending ?
Master the basic concepts of fluorescence at the level required to understand fluorometric assays and instrumentation.
Become familiar with the application of fluorometric assays to the major classes of pharmacological targets.
Understand the basis and applications of advanced methods such as fluorescence imaging, fluorescence polarization, time-resolved energy transfer, and fluorescence-fluctuation spectroscopy.
Learn the important types of interferences in fluorometric assays.
Discover where to find more advanced information, using the extensive list of references provided.
Fluorescence course topics:
Fluorescence fundamentals
Labels and labeling chemistries
Instrumentation
Interferences and limitations
Survey of principal fluorescence methods
Biochemical and cellular applications
Enzyme & Binding Assays in Drug Discovery
Afternoon of 9 September and all day
10 September 2008What will I gain by attending ?
Master the basic concepts underlying enzyme and binding assays
Learn how applications in drug discovery sometimes dictate assay optimization and interpretation which differ from textbook recommendations
Learn how best to extract the information you need from your assays
Enzyme & Binding course topics:
Enzyme kinetics
Enzyme inhibition: Competitive, noncompetitive, uncompetitive, irreversible, and promiscuous
Multiple-substrate enzymes
Binding equilibria and kinetics
Deviations from classical textbook behavior
Optimizing enzyme and binding assays for primary vs. secondary screening
Understanding mechanisms and extracting information from your data
Discussion of commercial data-analysis and simulation software
Case studies, including receptor binding, kinases, and proteases
Kaynak: http://www.selectbiosciences.com/conferences/Owicki_ICT2008/
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